Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale

GlycoMEV UR 4358 – Université de Rouen Normandie

Les 5^èmes Journées du GDR Chémobiologie, groupement de recherche CNRS, se sont déroulées les 5 et 6 juin 2025 à la Faculté de Pharmacie de Nantes et a réuni, comme chaque année, chercheurs, enseignants-chercheurs, ingénieurs et doctorants autour des dernières avancées en chémobiologie.

Au cours de ce rendez-vous d'échanges scientifiques et d'innovations, le Pr Arnaud LEHNER représentait le Laboratoire GlycoMEV et a donné une conférence intitulée :

« Glycans metabolic engineering for studying plant cell wall biosynthesis and functions. »
 

Résumé :

Glycans metabolic engineering is a powerful tool for studying the glycosylation of living cells [1, 2]. The use of modified monosaccharides, such as deoxy or fluorinated sugars, has been reported as a powerful pharmacological approach for studying carbohydrate metabolism and plant cell wall biosynthesis [3]. Moreover, non-natural metabolite derivatives that carry functions enabling bio-orthogonal ligations are now widely used for glycomolecules imaging in living organism. In both cases, these derivatives must cross the cell membrane and be accepted by the biosynthetic machinery of the cell to produce nucleotide-sugars that will be taken in charge by the enzymatic machinery to build complex glycomolecule such as cell wall polysaccharides in plant. Here, we illustrate the use of glycan metabolic engineering, photo release of caged monosaccharide derivative and click-chemistry, for studying the plant cell wall biosynthesis and function with an emphasis of the pectic domain rhamnogalacturonan II [4, 5].